The Tory leadership candidate has been accused of misconduct in public office after making the claim during the 2016 EU referendum campaign.
It is a private prosecution launched by campaigner Marcus Ball, who crowdfunded £200,000 for the case.
Mr Johnson's representatives have called the case a "stunt" that is being "brought for political purposes".
The preliminary hearing will take place at Westminster Magistrates' Court and the case will then be sent to the Crown Court for trial.
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